I'm not going to get too crazy over this list, as it is largely arbitrary and since its ign, Marvel is likely leaning on them. I will say that Black Cat should not be on here, and Harry Osborn being #6 is a total joke.
I cannot really argue with Doc Ock, but my fondness for the character has largely died because of this abomination of a storyline known as "The Superior Spiderman".
This list does seem to be based on who has the most intimate connection with Spidey, so because of that I'm largely ok with it. My list would simply be based on who I like more, as opposed to who is actually a greater foe of Spider-man.